The ACE C18-AR phase is a long-chain phenylalkyl phase, which adds phenyl (aryl) selectivity to C18-like retention. C18-AR offers hydrophobic, p–p, and dipole-dipole interactions with analytes, and shows increased retention for analytes with electron-withdrawing groups. It offers complementary selectivity to ACE C18, C18-PFP, C18-Amide, CN-ES, and SuperC18 phases, and is fully wettable with no organic modifier in the mobile phase in both isocratic and gradient modes. C18-AR has been shown to be an extremely stable and ultra-low bleed phase for LC-MS workflows, and is available in HPLC and UHPLC configurations, including nano, capillary, analytical, and preparative dimensions.
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Figure 1: ACE C18-AR can provide additional retention and selectivity in highly aqueous mobile phases for polar analytes
In this example, ACE CN-ES provides significantly more retention than the shorter chain cyanopropyl phase (ACE CN), and it offers comparable retention but different relative retention compared to the ACE C18 phase.
Figure 2: ACE C18-AR can deliver improved selectivity and resolution compared to C18 phases
Figure 3: Synthetic cannabinoids (SPICE compounds) in oral fluids are readily separated using ACE C18-AR and detected using LC-MS-MS