The ACE C18-PFP phase is a longer-chain pentafluorophenylalkyl phase, which adds pentafluorophenyl (PFP) selectivity to C18-like retention.  C18-PFP retains analytes via hydrophobic, p–p, dipole-dipole, hydrogen bonding, and steric (shape-selective) interactions, and shows increased retention for analytes with electron-donating groups.  C18-PFP is an extremely stable and ultra-low bleed phase for LC-MS analyses, and is available in HPLC and UHPLC configurations, including nano, capillary, analytical, and preparative dimensions.

ACE C18-PFP has complementary selectivity to ACE C18, C18-AR, C18-Amide, CN-ES, and SuperC18 phases, and is fully wettable with low or no organic modifier in the mobile phase. When used in a systematic method development scheme, these highly selective phases can make the separation of complex samples much more efficient and effective.

Advantages
  • Ideal column choice for method development when  C18 retention and alternate selectivity is needed
  • Complementary retention and selectivity to ACE C18, C18-AR, C18-Amide, CN-ES and SuperC18 bonded phases
  • Useful for analytes that contain electron-donating groups:  -NH2, -NR2, -OCH3, -OH, -alkyl, -phenyl/aryl
  • Compatible with 100% aqueous mobile phases and provides increased retention for polar analytes
  • Stable using low and mid pH eluents (pH 2–8)
  • Ultra-low bleed for UV-VIS and LC/MS compatibility
  • High efficiency 2 μm, 3 μm, 5 μm and 10 μm particles for UHPLC, HPLC and preparative separations

Figure 1: Catecholamines can be retained and separated under 100% aqueous conditions with no ion-pairing reagents on ACE C18-PFP

In this example, ACE CN-ES provides significantly more retention than the shorter chain cyanopropyl phase (ACE CN), and it offers comparable retention but different relative retention compared to the ACE C18 phase.

Figure 2: ACE C18-PFP can separate various nucleosides, nucleoside bases and B vitamins using 100% aqueous, isocratic conditions

Figure 3: This separation of aflatoxins is carried out isocratically using ACE C18-PFP and fluorescence detection



Care and Use Sheets Available on Request (email hplc@mac-mod.com)