The ACE C18-PFP phase is a longer-chain pentafluorophenylalkyl phase, which adds pentafluorophenyl (PFP) selectivity to C18-like retention. C18-PFP retains analytes via hydrophobic, p–p, dipole-dipole, hydrogen bonding, and steric (shape-selective) interactions, and shows increased retention for analytes with electron-donating groups. C18-PFP is an extremely stable and ultra-low bleed phase for LC-MS analyses, and is available in HPLC and UHPLC configurations, including nano, capillary, analytical, and preparative dimensions.
ACE C18-PFP has complementary selectivity to ACE C18, C18-AR, C18-Amide, CN-ES, and SuperC18 phases, and is fully wettable with low or no organic modifier in the mobile phase. When used in a systematic method development scheme, these highly selective phases can make the separation of complex samples much more efficient and effective.Advantages
Figure 1: Catecholamines can be retained and separated under 100% aqueous conditions with no ion-pairing reagents on ACE C18-PFP
In this example, ACE CN-ES provides significantly more retention than the shorter chain cyanopropyl phase (ACE CN), and it offers comparable retention but different relative retention compared to the ACE C18 phase.
Figure 2: ACE C18-PFP can separate various nucleosides, nucleoside bases and B vitamins using 100% aqueous, isocratic conditions
Figure 3: This separation of aflatoxins is carried out isocratically using ACE C18-PFP and fluorescence detection