ACE® CN-ES is a unique bonded phase that has an extended alkyl chain spacer with a terminal cyano group. ACE CN-ES provides more hydrophobic retention and stability than other cyano phases with shorter (usually n-propyl) spacers.
ACE CN-ES UHPLC and HPLC columns retain and separate analytes via dipole-dipole interactions and enhanced hydrophobic interactions, and offer alternative selectivity compared to other phases for polar analytes. ACE CN-ES columns can be used with 100% aqueous mobile phases in reversed-phase mode (RPLC), and remarkably, deliver excellent performance in normal-phase mode (NPLC).
The CN-ES phase provides alternate, orthogonal selectivity compared with other ACE phases such as ACE C18, ACE C18-AR, ACE C18-PFP, ACE SuperC18, and ACE C18-Amide. When used in a systematic method development scheme, these highly selective phases can make the separation of complex samples much more efficient and effective.
Figure 1 ACE CN-ES Provides Increased Retention and Alternative Selectivity
In this example, ACE CN-ES provides significantly more retention than the shorter chain cyanopropyl phase (ACE CN), and it offers comparable retention but different relative retention compared to the ACE C18 phase.
Figure 2 ACE CN-ES: Excellent for Method Development
The ACE CN-ES phase is an excellent addition to your set of orthogonal columns used for method development. It can often separate analytes that co-elute on other phases or can reverse analyte elution order.