HALO Fused-Core Columns have revolutionized HPLC, and now UHPLC, with HALO-2 Fused-Core Columns!
  • High speed and efficiency with 20, 30 and 50 mm columns
  • High resolution and peak capacity with 75, 100 and 150 mm columns
  • Improved productivity with faster analyses
  • Less solvent usage from shorter analysis times
  • Sharper, taller peaks = better sensitivity and lower LOD and LOQ
  • ~20% lower back pressure than most commercially available non-core sub-2-μm columns
  • Less subject to plugging because of 1 µm inlet frits
  • 300,000 plates per meter efficiency
HALO C18 & C8
Excellent performance for a broad range of analyte polarities. Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes.
HALO RP-Amide
Retention and selectivity due to both hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
HALO Peptide ES-C18
Ideal for ultrafast or high resolution separations of peptides and polypeptides ≤ 15 kDa. Enhanced stability at low pH via sterically protected ligands up to 90 degrees C.
HALO Phenyl-Hexyl
Separations occur due both to hydrophobic and π-π interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases.
HALO PFP
Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, π–π, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode with mobile phases containing > 80% acetonitrile. Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
Penta-HILIC
This unique bonded phase provides a highly polar stationary phase on which to perform HILIC separations. The high bonding density and polarity of the bonded phase minimize ion-exchange effects with residual silanols, thereby improving peak shape and lot-to-lot reproducibility.
HALO HILIC (silica)
Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
ES-CN
Provides strong dipole-dipole interactions with analytes and weak hydrophobic interactions. Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid equilibration with low bleed for high throughput LC-MS applications. Extra stable bonding with sterically protected ligand provides improved stability and performance compared to conventional CN phases, especially at low pH (1-4).

Figure 1 HALO 2 columns have a wide optimum flow rate range and outperform non-core columns in 1.7 μm particle size.

Figure 2 HALO 2 delivers improved performance compared to non-core sub-2-μm column for a separation of 14 explosives

Figure 3 Complex samples such as DNPH derivatives of aldehydes and ketones can be resolved quickly using HALO 2

Note: Small peaks adjacent to major peaks are geometric isomers of asymmetrical carbonyl compounds.

Care and Use Sheets Available on Request (email hplc@mac-mod.com)