HALO C18 & C8
Excellent performance for a broad range of analyte polarities. Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes.HALO RP-Amide
Retention and selectivity due to both hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
HALO Peptide ES-C18
Ideal for ultrafast or high resolution separations of peptides and polypeptides ≤ 15 kDa. Enhanced stability at low pH via sterically protected ligands up to 90 degrees C.
Separations occur due both to hydrophobic and π-π interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases.
Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, π–π, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode with mobile phases containing > 80% acetonitrile. Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
This unique bonded phase provides a highly polar stationary phase on which to perform HILIC separations. The high bonding density and polarity of the bonded phase minimize ion-exchange effects with residual silanols, thereby improving peak shape and lot-to-lot reproducibility.
HALO HILIC (silica)
Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
Provides strong dipole-dipole interactions with analytes and weak hydrophobic interactions. Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid equilibration with low bleed for high throughput LC-MS applications. Extra stable bonding with sterically protected ligand provides improved stability and performance compared to conventional CN phases, especially at low pH (1-4).
Figure 1 HALO 2 columns have a wide optimum flow rate range and outperform non-core columns in 1.7 μm particle size.
Figure 2 HALO 2 delivers improved performance compared to non-core sub-2-μm column for a separation of 14 explosives
Figure 3 Complex samples such as DNPH derivatives of aldehydes and ketones can be resolved quickly using HALO 2
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