HALO Protein and Peptide UHPLC columns are specifically designed for fast, high-resolution separation of proteins and peptides. These columns minimize the compromises that must be made between efficiency, speed of separation and column back pressure. The particles packed in these columns are engineered to have pore sizes, particle sizes and diffusion path lengths that are optimum for the separation of proteins up to 500 kDa (HALO Protein columns) and peptides in the range of 3 to 20 kDa (HALO Peptide columns).
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HALO 1000 Protein C4 >
Designed for fast, high resolution separations of intact monoclonal antibodies, antibody fragments, (including heavy and light chains) and large polypeptides and proteins up to 1.2 million kDa.
• 1000 Ångstrom pore size
• n-Butyldimethylsilane ligands, exhaustively endcapped and enhanced for high temperature stability up to 90 °C at low pH
• 2.7 µm particle size with 0.5 µm porous layer fused to 1.7 µm solid core
• Compatible with both UHPLC and HPLC up to 1000 bar

HALO 400 Protein ES-C18 >
• Designed for fast, high resolution separations of proteins and polypeptides having MWs < 500 kDa
• 400 Ångstrom pore size
• Sterically-protected octadecylsilane ligands with dense endcapping
• Extremely stable at low pH up to 90 degrees C
• 3.4 µm particle size with 0.2 µm porous layer fused to 3 µm solid core
• Compatible with UHPLC and HPLC up to 600 bar
HALO 400 Protein C4 >
• Designed for fast, high resolution separations of intact monoclonal antibodies, antibody fragments, (including heavy and light chains) and large polypeptides and proteins up to 500 kDa.
• 400 Ångstrom pore size
• n-Butyldimethylsilane ligands, exhaustively endcapped and enhanced for high temperature stability up to 90 °C at low pH
• 3.4 µm particle size with 0.2 µm porous layer fused to 3 µm solid core
• Compatible with both UHPLC and HPLC up to 600 bar

HALO Peptide ES-C18 >
• Designed for ultrafast and high resolution UHPLC and HPLC separations of peptides and polypeptides with 3 kDa ≤ MW ≤ 20 kDa
• 160 Ångstrom pore size
• Steric-protected octadecyldiisobutylsilane ligands
• Enhanced stability at low pH up to 90 degrees C, not endcapped
• 2.0, 2.7 and 5 μm particle sizes with 0.5 μm porous layers
• Compatible with UHPLC and HPLC up to 600 bar
HALO Peptide ES-CN >
• Provides an alternative selectivity to C18 columns for UHPLC and HPLC separations of peptides and polypeptides with 3 kDa ≤ MW ≤ 20 kDa.
• 160 Ångstrom pore size
• Steric-protected cyanopropyldiisopropylsilane ligands, highly endcapped
• Enhanced stability at low pH up to 90 degrees C
• 2.7 and 5 μm particle sizes with 0.5 μm porous layers
• Compatible with UHPLC and HPLC up to 600 bar

HALO Glycan >
• Ideal for hydrophilic interaction liquid chromatography (HILIC) separations of structurally diverse oligosaccharides—especially those of released and labeled glycans from monoclonal antibodies, glycoproteins and proteoglycans
• Superior retention and selectivity for highly sialylated glycans
• 90 Ångstrom pore size
• Highly polar, polyol ligand tethered via a novel, proprietary linker to Fused-Core particle
• Stable up to 65 degrees C
• 2.7 μm particle size with a 0.5 μm porous layer
• Compatible with UHPLC and HPLC up to 600 bar
Care and Use Sheets Available on Request (email hplc@mac-mod.com)