Partisil is a high-purity irregular silica gel available in both 5µm and 10µm particle sizes with a pore size of 80Å. The choice of column packing includes Silica, C-18 polymeric phases (ODS-3,ODS-2) and C-8. Also available are SAX, SCX, and PAC. These columns provide reproducible results, column to column, lot to lot.
Normal Phase (Adsorption) Media
Partisil Silica (5 and 10µm)
Pure silica stationary phase for adsorption chromatography. Partisil 10 is used particularly for routine separations for higher flow rates and lower back pressures. Partisil 5 is used particularly for higher resolution and fast analysis. These are the supports on which the Partisil bonded phases are based.
Ion Exchange Media
Partisil SAX (5 and 10µm)
A strong anion exchanger based on quaternary ammonium groups (-NR+3 ). Supplied in the H2PO4 form in methanol, Partisil 10 SAX has been widely reported in literature and is best known for the separation of nucleotides. Stable over pH range 1.5-7.5 when used in conjunction with a Solvecon mobile phase conditioning column. Obtains the highest anion exchange efficiencies and resolution. Applicable to separations of nucleic acids, organic acids and inorganic anions.
Partisil SCX (5 and 10µm)
Based on aromatic benzene sulfonic acid groups. Supplied in the ammonium form. Excellent for separation of nucleosides, amino acids, polyamines, drugs and other cationic species. Capable of being loaded with specific metallic cations for use in ligand exchange chromatography. Stable over pH range 1.5-7.0 when used in conjunction with a Solvecon mobile phase conditioning-column. Exceptionally stable Si-O-Si-C bond, both thermally and chemically.
Reverse Phase Media
Partisil ODS (10µm)
A C-18 phase with a 5% carbon load for both normal adsorption and reversed phase partitioning. Dual-mode operation for added selectivity with 50% residual silanols. Lightly loaded C-18 packing is particularly effective for compounds having greater water solubility when used in the reversed phase mode. Creates a moderately polar surface, different from that of pure silica, in normal phase mode.

Partisil ODS-2 (10µm)
The high carbon load (16%) of this polymeric phase makes it the most nonpolar and, therefore, the most retentive of the reversed phases. An alternative to end-capped C-18 where different elution order is desirable for optimum separation. High sample load capacity and 10 ┬Ám particle size are very suitable for preparative work.

Partisil ODS-3 (5 and 10µm)
A C-18 polymeric phase with a 10.5% carbon load. Medium of choice for improved speed, efficiency and resolution in applications requiring C-18 phases. End-capped for deactivation of silanols to minimize the need for ion suppression or ion pairing agents. Used in a wide range of applications with optimal selectivity, including pharmaceuticals, natural products, food, biological and environmental pollutants.
 
Partisil C8 1(5 and 10µm)
An end-capped C-8 monomeric phase with at least 8.5% carbon load. Provides high efficiency and rapid mass transfer while maintaining excellent peak shape and stability over a range of aqueous mobile phase compositions. Recommended for ion pair chromatography.

Partisil PAC (5 and 10µm)
A polar amino cyano bonded phase with secondary amine groups for good thermal and chemical stability. Selectivity and rapid equilibrium allow a range of separation mechanisms to be used, including adsorption, reversed phase and weak anion exchange. Extremely fast equilibration across the entire range of solvents from heptane to water. The media of choice for carbohydrate separations.
Care and Use Sheets Available on Request (email hplc@mac-mod.com)