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The Role of Methanol and Acetonitrile as Organic Modifiers in Reversed-Phase Liquid Chromatography

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Gradient Chromatography

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UHPLC Fittings and Connections

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The Fundamental Resolution Equation and the Impact of k, N and α

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Basic Column Care and Cleaning

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How to Calculate Resolution

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Retention Factor, k

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ProntoSil Catalog

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HALO® AQ-C18 Catalog

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Examination of PFAs Analysis Using LCMS for EPA Methods 537.1 and EPA 8327

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Using Selectivity of Fused-Core Particles to Maximize Resolution for Improved LC and LC-MS Method Development

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Guidebook on Reversed Phase Chemistries & Utilizing Selectivity for HPLC Separations

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90 A Selectivity Kit Cards

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Improve Your Analyses of Large Proteins, Antibodies and Antibody-Drug Conjugates Using Reversed-Phase Liquid Chromatography by Maximizing Pore Access

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HALO® Catalog

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Analyses of Large Proteins, Antibodies, and Modified Proteins Using A New Series of Advanced Large Pore HPLC Materials

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Ion-Pair Reversed-Phase Analysis of an Oligonucleotide Ladder Standard

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Glycan Analysis Using HILIC with Fluorescence Detection

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Peptide and Protein Guide by David Carr

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Avantor® ACE® UltraCore Buffer Recommendations

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Avantor® ACE® Method Development Kit Catalog

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HILIC Method Development Guide

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The Importance of Sample Diluent in HILIC

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Column Equilibration in HILIC Mode

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Avantor® ACE® HILIC Brochure

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An Intelligent Solution for Hydrophilic Interaction Liquid Chromatography Method Development

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A Simple Step by Step Protocol for HILIC Method Development

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UHPLC Guard Columns

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The Power of Stationary Phase Selectivity

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Streamlined Method Development Using Column Screening with Advanced Computer Simulation Software

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A Step by Step Protocol for Streamlined Reversed-Phase Method Development

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Method Development for the Separation of Major Cannabinoids and Terpenes Using a Six Column Selectivity Screening Approach

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Understanding and Overcoming Separation Challenges in Biological Drug Development

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Selectivity and Rapid Method Development, Method Translations, and Instrument Transfers

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Improvement of Both Legacy and New HPLC Methods with Superficially Porous Particle Columns

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An Introduction to the Theory and Practice of LC Method Translations and LC Method Transfers

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Sensitive and Selective Quantification of Microcystin Toxins in Drinking Water by UHPLC-MS-MS

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A UHPLC-MS-MS Method for the Separation and Low-Level Determination of Catecholamines and Metanephrines in Urine

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A Multi-Residue LC-MS-MS Method for the Trace Analysis of 300 Pesticides Using a Solid-Core Stationary Phase With Unique C18 Bonding Chemistry

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High Performance Separations Using a Novel Bonded Phase Compatible With 100% Aqueous Mobile Phases

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Molecular Probes to Characterize HPLC Column Performance

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Protein Separations Using High Performance Large Pore Superficially Porous Particles Optimization Strategies for mAb Variant Resolution

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Development of a Method for the Separation of Major Cannabinoids Using Six Column Selectivity Screening

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Wide Pore Superficially Porous Particles with Several Bonded Phases for High Resolution Protein Chromatography

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Characterization of the Activity and Kinetics of Guanine Deaminase

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High Resolution Separations for Detailed LC-MS Analysis of mAb Disulfide Variants

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Protein Separations Using Large Pore Superficially Porous Particles

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Accelerating HPLC and UHPLC Method Development With Selectivity Influence of Column Chemistries

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A Useful Method Development Strategy For Larger Protein Variant RPLC Separations

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LC-MS Method for Analysis of Guanine Deaminase, Activity, Kinetics and Effects of Inhibitors

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Know Your Instrument, Know Your Column, Know Your Peace – For Method Development and Transfer

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Streamlined Reversed Phase Method Development using a Combined Column Screening and Software Modeling Approach

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New Reversed-Phase SPP Columns with Alternate Selectivity for Small Molecules

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Optimizing HPLC Separation Performance for Peptides and Other Mid-Size Molecules

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Wide Pore Superficially Porous Particles with Various Bonded Phases for High Resolution Protein Chromatography

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All You Wanted to Know about Method Development and Transfer, but Were Afraid to Ask

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One Size Does Not Fit All, Exploring the Relationship Between Pore Size and Separation Efficiency

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LC-MS Method for the Analysis of Guanine Deaminase

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High Performance Separations Using 100% Aqueous Mobile Phase Compatible Superficially Porous Particle Columns Coupled with Mass Spectrometry

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A Diphenyl Bonded Phase on Wide Pore Superficially Porous Particles For Efficient Separations of Proteins

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Selectivity Manipulation for LC-MS Analysis of Protein Variants

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Separation and Low-Level Determination of Catecholamines and Metanephrines from Urine by UHPLC-MS-MS

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Hydrophilic Interaction Liquid Chromatography (HILIC) Method Development Protocol

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Investigating the Effects of Chromatographic Parameters on Column Equilibration in Isocratic and Gradient HILIC Separations

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Low-Level LC-MS-MS Determination of Amphetamines and Opiates From Urine

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A Streamlined Approach for Reversed-Phase Method Development Using a Combination of Column Screening and Software Modeling

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Column Pore Size – An Underutilized Variable for Optimizing HPLC Separations

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New Superficially Porous Particle Column Selectivity Choices for Improved Separations

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Improving Larger Protein Separations Using 1000 A Superficially Porous Particles

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Developing HPLC Methods When C18 Columns Don’t Work

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An Interactive Spreadsheet-based Approach to Simplify Method Transfer Between Different Vendor UHPLC and HPLC Instruments

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A Practical, Selectivity Based HILIC Method Development Protocol

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Comparison of Phenyl and C18 Bonded Phases with Peptide Mixtures

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Size Exclusion Chromatography (SEC) with Superficially Porous (Core-Shell) Particles

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Superficially Porous Particles with Polar C18 Selectivity

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An Effective Method Development Strategy Using Fused-Core Columns

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Effects of Eliciting Immune Response in Rabbits on IgG N-Glycans

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Profiling the N-Glycosylation of BioTherapeutic IgGs with HILIC-MRM Analysis of Trypsin Digested Culture Media

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Comparison of Phenyl and C18 Bonded Phases to Obtain Separation Selectivity of Peptide Mixtures

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High Efficiency Wide Pore Superficially Porous Particles for LC-MS of Larger Proteins

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Improving the Separation and MS Detection of Proteins and Protein Fragments Using New Mobile Phase Options in Reversed-Phase Chromatography

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Accelerating UHPLC and HPLC Method Development Strategies by Leveraging Selectivity

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A Step by Step Approach for Method Development to Generate a Successful HILIC Separation

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Low Level Determination of Catecholamines, Epinephrine, Norepinephrine and Dopamine from Plasma by UHPLC-MS-MS

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Rapid Separation and Determination of 25-Hydroxy Vitamin D2, D3 in Serum by UHPLC-MS-MS

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Translating US Pharmacopoeia Methods to Sub-2 Micron and Solid Core Using the New USP (621) General Chapter Guidelines

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Translating Natural Product Methods to Multiply Coupled Columns to Achieve Ultra-Resolution Separations and the Potential Impact of Pressure Induced Changes in Selectivity

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Practical Method Development and Optimization for UHPLC and HPLC Separations of Peptides for Peptide Mapping Analyses Using Different Mobile Phase Additives

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Impact of Pore Exclusion on RP-HPLC Column Performance

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LC-MS of mAb Structure Utilizing HALO® BioClass Fused-Core® Particles

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Improving Superficially Porous Particles for Larger Protein Separations