Abstract
The use of different pore sizes and pore volumes and the impact this has on separation performance, as well as the column loading capacity will be discussed. This will be done for a range of different molecular mass molecules and the impact of pore size and pore volume will be demonstrated, with a particular focus on the separations of biomolecules.
Example separations will include intact and partially digested mAbs, as well as a full tryptic digest. The protocols for the digestion will be discussed and the impact of different column chemistries will be investigated to demonstrate the importance of assessing a range of column chemistries during method development, even for large biomolecule separations.