Abstract
An ongoing effort to modernize USP methods for HPLC analysis is in progress. There are criteria in effect which allow changes in column without having to revalidate the method. For those running legacy HPLC methods, switching to superficially porous particle columns offers several advantages including reduced analysis time/higher throughput, increased efficiency, and improved resolution. In this webcast, we will highlight case studies that compare existing methods using fully porous particle columns to separations using superficially porous particle columns. When developing new methods, the choice of which stationary phase to select can also be challenging. Using a strategy to screen several different stationary phases when developing methods can be more effective than just starting with a C18 column. The second part of this webcast will show example comparisons across various stationary phases. Whether an analyst is looking to improve an existing method or developing a new method, employing superficially porous particle columns will enable fast, efficient separations.