Abstract
Reversed-phase method development can be a very time-consuming and demanding task, particularly if your samples contain many analytes, and if you don’t follow a systematic approach. In industrial and pharmaceutical research laboratories, researchers often must develop analytical methods that require the separation of tens of components including active pharmaceutical ingredients (APIs), process impurities, API degradants, formulation excipients, as well as degradants resulting from chemical reactions between APIs and excipients.
In this webinar, we will discuss the usefulness of RPLC stationary phase and mobile phase screening and will show how it can be effective for selecting combinations for further development and refinement. We will also review some tools and calculations that are useful in experimental planning, delay volume and extra-column volume calculations.