Abstract
The first half of this webinar will focus on chromatographic selectivity and how it can be exploited by adjusting key method parameters to maximize resolution for various analyte mixtures and classes. This will include optimizing analytical method development workflows to reduce development time, but maintain critical peak pair resolution. This rationale will be applied to an HPLC / UHPLC method development case study for a pharmaceutical-related substances analysis.
The second half of the webinar will review HPLC/UHPLC isocratic and gradient method translation activities with example pharmaceutical assay and related substances methods. Highlights of the new USP <621> guidance regarding changes to chromatographic methods will be included. Method translations between different particle sizes and column geometries, including totally porous and solid-core particle platforms will be discussed, and differences between these situations will be highlighted. Finally, maximizing resolution for complex natural product samples will be illustrated using solid-core particle columns.